human tnbc cell lines hs 578 t  (ATCC)

Journal: Cell Death Discovery

Article Title: MSLN-mediated activation of EGFR-ERK1/2 signaling drives liver metastasis in breast cancer

doi: 10.1038/s41420-025-02835-9

Figure Lengend Snippet: A Pearson correlation analysis of the TCGA-TNBC dataset revealed a positive correlation between MSLN and ELF1 expression. B JASPAR database analysis identified the ELF1 recognition motif in the MSLN promoter (upper), and a schematic diagram (lower) illustrated potential ELF1-responsive elements (E1), and introduced an E1 sequence mutation (WT: CAGGAGGAG; Mut: ACAACAACA). C Luciferase reporter assay showed E1-dependent transcription regulation of MSLN by ELF1 in 293 T and HCC1806 Pri cells. D ChIP assays were performed to validate ELF1 binding to the MSLN promoter in 293 T and HCC1806-derived cells. ELF1 regulation of MSLN was analyzed via Luciferase reporter assay ( E ) qRT-PCR ( F ) and WB ( G ) in HCC1806 and 4T1-derived cells. H Pearson correlation analysis of ELF1 and MSLN expression in TNBC patients below median overall survival. (Data are presented as the mean ± SD; ns no statistical difference, * P < 0.05, *** P < 0.001).

Article Snippet: Human TNBC cell lines (HCC1806, MDA-MB-231, Hs578T, BT-549) and mouse breast cancer cell lines (EMT6, 4T1, E0771, PY81119) and 293 T cell line were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Expressing, Sequencing, Mutagenesis, Luciferase, Reporter Assay, Binding Assay, Derivative Assay, Quantitative RT-PCR

Journal: Cell Death Discovery

Article Title: MSLN-mediated activation of EGFR-ERK1/2 signaling drives liver metastasis in breast cancer

doi: 10.1038/s41420-025-02835-9

Figure Lengend Snippet: The transcription factor ELF1 in hepatotropic metastatic cancer cells causes an increased MSLN. The elevated MSLN interacts with EGFR, resulting in phosphorylation of EGFR as well as the activation of downstream ERK1/2 signaling pathway, thereby promoting disseminated TNBC cell survival and proliferation, thus lead to liver metastasis.

Article Snippet: Human TNBC cell lines (HCC1806, MDA-MB-231, Hs578T, BT-549) and mouse breast cancer cell lines (EMT6, 4T1, E0771, PY81119) and 293 T cell line were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Phospho-proteomics, Activation Assay

Journal: BMC Pharmacology & Toxicology

Article Title: Integrated in silico and in vitro evaluation of Genistein and Apigenin as dual inhibitors of PARP1 and ESR1 in breast cancer

doi: 10.1186/s40360-025-01082-z

Figure Lengend Snippet: Dose-dependent cytotoxic effects of GNT and Apigenin on HCC1937 cells evaluated by the MTT assay. ( A ) Dose–response curves showing percentage cell viability plotted against the logarithm of compound concentration (µM). ( B ) Bar graph representation of cell viability at increasing concentrations (10–400 µM) compared to control. Data are presented as mean ± SD ( n = 6)

Article Snippet: HCC1937 human triple-negative breast cancer (TNBC) cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA; Cat. No. CRL-2336).

Techniques: MTT Assay, Concentration Assay, Control